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	Provedor de dados BJMBR (1) Rev. Microbiol. (1) Electron. J. Biotechnol. (1) Autor Bernardo-Filho,M. (1) Carvalho,Cibele B. Mano de (1) Chiang,Yawen L (1) Chin,Emily (1) Ferreira,Maria Candida S. (1) Freitas,R.S. (1) Huang,Li-chun (1) Lima-Filho,G.L. (1) Moreira,José Luciano B. (1) Moreno,S.R.F. (1) Rocha,E.K. (1) Mais... Palavra-chave Plasmid DNA (3) Antimicrobial resistance (1) Bacteroides fragilis group (1) Blood element labeling (1) Cell proliferation (1) Ginkgo biloba extract (1) Potency assays (1) Receptor binding (1) Stannous chloride (1) Technetium-99m (1) VEGF-2 (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Journal article (1) Ano 2010 (1) 2004 (1) 1998 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Development of potency assays for a plasmid containing vascular endothelial growth factor 2 Electron. J. Biotechnol. Huang,Li-chun; Chin,Emily; Chiang,Yawen L. We have developed analytical methods to measure the biological functions of pVGI.1(VEGF2), a naked plasmid DNA product containing vascular endothelial growth factor 2 used in clinical trials for coronary artery diseases (CAD) and peripheral artery diseases (PAD). After being injected into muscles, vascular endothelial growth factor 2 (VEGF-2), presumably expressed in muscle tissues, binds to the endothelial cell receptors VEGFR2 or VEGFR3, triggering the downstream responses including cell proliferation and vascularization. As it is important to make sure clinical material is biological active, we developed a quantitative assay first to measure the receptor binding activity of the pVGI.1(VEGF2) gene product expressed by the transfected host cells, and then... Tipo: Journal article Palavras-chave: Cell proliferation; Plasmid DNA; Potency assays; Receptor binding; VEGF-2. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100001 Protection of plasmid DNA by a Ginkgo biloba extract from the effects of stannous chloride and the action on the labeling of blood elements with technetium-99m BJMBR Moreno,S.R.F.; Freitas,R.S.; Rocha,E.K.; Lima-Filho,G.L.; Bernardo-Filho,M.. Ginkgo biloba extract (EGb) is a phytotherapeutic agent used for the treatment of ischemic and neurological disorders. Because the action of this important extract is not fully known, assays using different biological systems need to be performed. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and used in nuclear medicine. The labeling depends on a reducing agent, usually stannous chloride (SnCl2). We assessed the effect of different concentrations of EGb on the labeling of blood constituents with Tc-99m, as sodium pertechnetate (3.7 MBq), and on the mobility of a plasmid DNA treated with SnCl2 (1.2 µg/ml) at room temperature. Blood was incubated with EGb before the addition of SnCl2 and Tc-99m. Plasma (P) and RBC were separated and... Tipo: Info:eu-repo/semantics/other Palavras-chave: Ginkgo biloba extract; Blood element labeling; Technetium-99m; Stannous chloride; Plasmid DNA. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200015 Transfer of clindamicyn resistance between Bacteroides fragilis group strains isolated from clinical specimens Rev. Microbiol. Carvalho,Cibele B. Mano de; Moreira,José Luciano B.; Ferreira,Maria Candida S.. Clindamycin resistance was trasferred by a conjugation-like process from Bacteroides thetaiotaomicron 52, a multiple antibiotic-resistant strain isolated from clinical specimens, to other Bacteroides species. A possible association between a plasmid detected in the donor strain and clindamycin resistance is discussed. Tipo: Info:eu-repo/semantics/other Palavras-chave: Bacteroides fragilis group; Antimicrobial resistance; Plasmid DNA. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300007 Registros recuperados: 3 Primeira ... 1 ... Última

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